Fig 1.
A genomic position of the BAC (ID: CH17-24F19).
The BAC contains five genes: caspase recruitment domain family member 17 (CARD17), solute carrier family 26 member 11 (SLC26A11), ring finger protein 213 (RNF213), N-sulfoglucosamine sulfohydrolase (SGSH), CTD-2047H16.4 (uncharacterized gene).
Fig 2.
An overview of the BAC double capture (BDC) method, which we modified (Maricic et al., 2010) [7].
On the left, the bait construction from the BAC is shown; on the right, the production of indexed libraries that are used in the library enrichment (center). Those colored light red are the BAC-based baits, dark red represents targeted DNA molecules in the libraries, black represents non-targeted DNA molecules in the libraries, green and pink represent indexes, gray represents adapters, and blue and yellow represent biotinylated adapters. Thick lines represent double stranded DNA, and thin lines represent single stranded DNA.
Fig 3.
On-target rates depending on BAC baits length.
On-target rate equals the reads mapped to the target region divided by the reads mapped to the whole reference genome.
Fig 4.
On-target rates depending on the number of captures.
The formula to calculate the on-target rate is the same as in Fig 3.
Fig 5.
On-target rates of the hybridization temperature.
The formula to calculate the on-target rate is the same as in Fig 3.
Table 1.
Average numbers of BDC and MB.
Table 2.
Numbers of validated SNPs.
Table 3.
Concordant rates of genotypes.
Table 4.
Summary of comparisons between BDC and MB.