Fig 1.
The newly identified wheat miRNAs’ secondary structures.
The wheat pre-miRNAs’ secondary structures were developed through the Mfold algorithm. These structures clearly show the mature miRNAs in green, in the stem portion of the stem-loop structures.
Fig 2.
Wheat miRNAs RT-PCR expressional validation.
One housekeeping gene and nine wheat miRNAs; 1 (Housekeeping gene Ta54227- AAA-superfam. ATPases), 2 (tae-miR5040), 3 (tae-miR6220), 4 (tae-miR169), 5 (tae-miR172d), 6 (tae-miR827), 7 (tae-miR5523), 8 (tae-miR530b), 9 (tae-miR530a) and 10 (tae-miR1522), were selected and subjected to RT-PCR expression analysis for the experimental validation. The product of each sample was separated on a 1.5% (w/v) agarose gel with 100 base pair DNA leader.
Table 1.
Putative wheat targets enrichment analysis in GO-terms.
Where, BP = Biological Process, MF = Molecular Function, CC = Cellular Component, and FDR = False Discovery Rates.
Fig 3.
Based on agriGo, more complicated enriched biological processes was built and wheat miRNAs are involved in many different biological processes, mainly in reproduction, multi-organism process, cell communication, cell recognition, respiration and biosynthesis processes.
Fig 4.
Based on agriGo, more complicated enriched cellular component processes was built and wheat miRNAs are involved in many different cellular components, mainly in membrane and membrane-linked, mitochondrion and small ribosomal subunit.
Fig 5.
Based on agriGo, more complicated enriched molecular functional processes was built and wheat miRNAs are involved in many different molecular functions, mainly in bindings, nutrient reservoir activity, hydrolase activity and in RNA polymerase activity.