Fig 1.
Experimental setup.
Fig 2.
Timeline of the experiment.
Fig 3.
Photos of (A) experimental arrangement for in vivo tests. (B) In vivo application of plasma jet.
Table 1.
Scores for epithelial alterations.
Fig 4.
Typical current and voltage waveforms for 2.0 slm of He flow and 1.5 cm distance to the target.
Fig 5.
Q-V Lissajous figure for one voltage cycle.
The figure’s area is 151.3 μJ.
Fig 6.
Number of viable cells in C. albicans ATCC 18804 biofilms (median and range) exposed or not to AM-CAPPJ (n = 9).
* p<0.05.
Fig 7.
Vero cells viability (mean percentage and standard deviation) after exposure to AM-CAPPJ.
Cell viability was measured immediately, 24 and 48 hours after the exposure to plasma jet (n = 6).
Fig 8.
Microscopic images of dorsal surface of C. albicans-infected tongues 24 hours after AM-CAPPJ exposure.
(1) HE-stained; (2) PAS-stained; (A) candidiasis induced and non-treated group; (B) plasma jet-treated; (C) nystatin-treated; (D) Scores (median values and ranges) attributed to the inflammatory alterations (column 1) and tissue invasion (column 2) 24 hours after exposure to AM-CAPPJ.
Fig 9.
Microscopic images of dorsal surface of C. albicans-infected tongues 48 hours after AM-CAPPJ exposure.
(1) HE-stained; (2) PAS-stained; (A) non-treated group; (B) plasma-treated; (C) Nystatin-treated; (D) Scores (median values and ranges) attributed to the inflammatory alterations and tissue invasion 48 hours after exposure to AM-CAPPJ.
Fig 10.
Counts of colony forming units in mice’ tongues 24 hours after the treatment with nystatin or AM-CAPPJ.