Table 1.
Topographical characterization (profile roughness parameters).
Table 2.
Topographical characterization (areal parameters).
Fig 1.
3D maps and related 2D profiles.
3D maps and related 2D profiles of: a) machined, b) anodized, c) TiN-coated, and d) ZrN-coated disks.
Fig 2.
Proliferation of fibroblasts seeded on Ti disks.
(a) MTT assay of HGFs cultured on the different Ti disks for 3, 7, 14 and 21 days shows that cells are vital and able to proliferate on all the surfaces examined. (b) SEM images (1000x magnification) of HGFs grown onto the four Ti disks reveal that cells reached confluence after 14 days from seeding, forming a continuous monolayer, which persisted up to 21 days.
Table 3.
Hemolysis test.
Table 4.
Ames test.
Fig 3.
Real-time PCR analysis of cell adhesion and proliferation markers in HGFs cultured on Ti disks for 21 days. Results for each experiment are obtained from triplicate experiments and values are expressed as the mean ± SD.
Fig 4.
IF staining of HGFs grown on Ti disks for 14 days. Upper panels display staining of vinculin (in green); lower panels show labeling of actin filaments by Phalloidin (in red). For both panels, nuclei are stained blue. Images taken at 40x magnification.
Fig 5.
Percentage of dead bacteria in five strains grown on disks after 120 hours of incubation: a) S. oralis; b) S. salivarius; c) S. sanguinis; d) S. mutans; e) S. sobrinus.