Fig 1.
Synthesis conditions of sialyllactuloses by TLC analysis.
L, C, N in every figure were standards. L, lactulose; C, CTP; N, Neu5Ac; (a), synthesis of Neu5Ac-α2,3-lactulose at different pH; (b), synthesis of Neu5Ac-α2,6-lactulose at different pH; (c), synthesis of Neu5Ac-α2,3-lactulose and Neu5Ac-α2,6-lactulose for different time; (d), synthesis of Neu5Ac-α2,3-lactulose and Neu5Ac-α2,6-lactulose at different temperatures. In Fig 1C and 1D, in the vertical bar on the left is reaction of Neu5Ac-α2,3-lactulose, on the right is reaction of Neu5Ac-α2,6-lactulose.
Fig 2.
Mass spectrum of purified Neu5Ac-α2,3-lactulose and Neu5Ac-α2,6-lactulose.
Fig 3.
The bacterial growing curves of S. aureus in modified medium containing α2,3-linked and α2,6-linked sialyllactuloses.
The control strain was not added sialyllactulose. The results are the mean values obtained from three separate experiments. Error bars represent the standard deviation.
Fig 4.
Changes of fluorescence intensity of S. aureus strains.
a, b, c and d were the samples with different carbohydrates. a, control; b, lactulose; c, α2,3-linked sialyllactulose; d, α2,6-linked sialyllactulose.
Fig 5.
Changes of DNA content of S. aureus with α2,3-linked and α2,6-linked sialyllactuloses.
Fig 6.
Protein leakage in S. aureus cells exposed to α2,3-linked and α2,6-linked sialyllactuloses.