Fig 1.
Illustration of central vein, portal tract and perisinusoidal regions in H&E stained image and SHG/TPEF image.
H&E: hematoxylin & eosin, SHG: second harmonic generation, TPEF: two-photon excited fluorescence.
Fig 2.
Illustration of specific collagen distribution at central vein, portal tract and perisinusoidal regions in early versus advanced fibrosis.
Fig 3.
Illustration of collagen parameters on SHG microscopy.
A) The RAW SHG/TPEF image. (B)The high magnification imaging performed in the area delimited by the blue square in picture A. (C) The aggregated and distributed strings of picture B. (D) The high magnification imaging performed in the area delimited by the blue square in picture B, and illustration of the width, length and area of a string. The thick string is by definition length-width ratio > 0.25.
Table 1.
Baseline characteristics of NAFLD cohort.
Fig 4.
Boxplot of SHG B-index and Brunt fibrosis stage (n = 83).
Table 2.
AUROCs of SHG B-index for prediction of Brunt fibrosis.
Fig 5.
AUROCs of SHG B-index to predict fibrosis stages.
Table 3.
Cross-validation of SHG B-index to predict Brunt fibrosis stage.