Table 1.
Scores attributed to the formation and quality of bone tissue.
Table 2.
Expressed quantities of minerals in EBS detected by ICP-OES (g/kg).
Table 3.
Expressed quantities of mineral contaminants in EBS detected by ICP-OES (g/kg).
Fig 1.
Surface characterization using SEM analysis of EBS at X2.500 (A) and X5.000 (C); and Bio-Oss® at X2.500 (B) and X5.000 (D).
Table 4.
Distribution of the animals according to the scores attributed to formation and quality of bone tissue.
Table 5.
Neoformed bone expressed as percentage of total defect area (mean ± SD).
Fig 2.
Histological findings of bone defect after 21 days.
A and B represent the -C group with the presence of osteocytes (black arrow), osteoblasts (white arrow) and neoformed bone trabeculation (NB). C and D represent the +C group showing mesenchymal tissue (MT) and discret neoformed bone trabeculation (NB). E and F represent the EBS group where the predominance of MT and multifocal points of NB occupy the bone defect, presence of osteocytes and osteoblasts. B indicates Bio-Oss®; EBS indicates experimental bone substitute; * indicates the border of the bone defect. Tissues were stained with H&E and examined at 5x and 20x magnification (200 μm and 50 μm, repectively).
Fig 3.
Bone formation score median between groups at different periods.
Different symbols (*,**) indicate statistically significant differences between groups at the same period.