Table 1.
Primer sequences for amplifying GATA3 and STAT4 genes to study rs3824662 and rs10181656 polymorphisms.
Table 2.
The demographic, anthropometric and clinical characteristics of Type 2 diabetic patients and controls.
Fig 1.
Agarose gel electrophoresis of PCR amplicons of the GATA3 gene containing 691 base pairs amplified with outer primers and 506 base pairs amplified with allele specific primers.
Each lane had the common 691 base pair band amplified with outer primer pair. Lanes 1 and 2 indicate the heterozygous genotype (GT), as both inner primers produced PCR products in two different reaction tubes with the same sample. Lanes 3 to 14 indicate the genotype of six samples as homozygous wild type (GG). Lanes 16 and 17 clearly indicate the sample that represents mutant homozygous type (TT), as only the T allele-specific inner primer produced a band of 506 base pairs. Lane 15 indicates the 100 base pair ladder.
Table 3.
Distribution of genotypic frequencies with respect to the GATA3 gene in Type 2 diabetic patients and healthy individuals.
Table 4.
Distribution of genotypic frequencies with respect to the GATA3 gene in male type 2 diabetic patients and healthy individuals.
Table 5.
Distribution of genotypic frequencies with respect to the GATA3 gene in female Type 2 diabetic patients and healthy individuals.
Fig 2.
Agarose gel electrophoresis of PCR amplicons amplified for the STAT4 gene comprising 157 base pairs.
Lanes 1, 2, 3, 4, 6, 7, and 8 designate PCR products of template DNA from study participants, and lane 9 designates a negative control in which no DNA was used as a template. Lane 5 indicates a 100 base pair DNA ladder.
Fig 3.
Agarose gel electrophoresis of DdeI digested products of PCR amplicons of STAT4.
Lane 11 contains the negative control. Here, DNA fragments of 157, 102, and 55 base pairs were found. Lanes 3, 6, 8, and 9 represent undigested bands of 157 base pairs, which represent the wild-type CC homozygote. Lanes 1, 2, 7, and 10 represent bands of 157, 102, and 55 base pairs that clearly indicate the presence of the heterozygous CG allele. Lane 4 represents completely digested bands of 102 and 55 base pairs that indicate the mutant GG allele. Lane 5 indicates the 100 base pair ladder.
Table 6.
Distribution of genotypic frequencies with respect to the STAT4 gene in Type 2 diabetic patients and healthy individuals.
Table 7.
Distribution of genotypic frequencies with respect to the STAT4 gene in male Type 2 diabetic patients and healthy individuals.
Table 8.
Distribution of genotypic frequencies with respect to the STAT4 gene in female Type 2 diabetic patients and healthy individuals.