Table 1.
Clinical parameters of patients included in the study.
Fig 1.
The number of proteins identified in declining order of patients.
This figure shows the distribution of 314 proteins identified in perilymph samples from fifteen patients. Sixty proteins were identified in all samples, and 91 in 12 or more samples. Note that 130 proteins were only identified once in 15 patients. Mass spectrometer data was analyzed using MaxQuant software.
Table 2.
Univariate linear regression results for proteins vs. tumor-associated hearing loss.
Fig 2.
Boruta analysis for the outcome tumor-associated hearing loss.
In brief Boruta analysis identifies variables performing better than random variables based on repeated Random Forest analysis. The shadow value represents random data. Boruta analysis showed that Alpha-2-HS-glycoprotein (P02765) was stronger than random data, while the four proteins identified as significant by univariate linear regression (P01857, P01834, P01024 and P01860) were rejected.
Fig 3.
PANTHER overrepresentation test of biological processes.
Orange bars represent the proteins detected in the perilymph while gray bars represent the expected number of proteins in each biological process group. The scale is logarithmic. In total, perilymph proteins were involved in 38 up-regulated biological processes while involved in two down-regulated biological processes.
Fig 4.
PANTHER overrepresentation test of molecular function.
Radar diagram, with a logarithmic scale, showing a comparison between the numbers of expected and detected proteins in each molecular function group. Orange boxes represent the detected number while gray triangles show the expected number. In total, 12 enriched molecular functions and one down-regulated.
Fig 5.
PANTHER overrepresentation test of cellular component.
Radar graph with a logarithmic scale showing a comparison between the numbers of expected and detected proteins in each cellular component group. Within the perilymph (orange boxes), nine functions were up-regulated compared to expected levels (gray triangles) while two were down-regulated.
Fig 6.
PANTHER overrepresentation test of PANTHER pathways.
Image shows a logarithmic radar graph comparison between the detected (orange boxes) and expected (gray triangles) numbers of proteins in each group. Seven significantly up-regulated PANTHER pathways were found.
Fig 7.
PANTHER overrepresentation test of PANTHER protein class.
Image shows a logarithmic radar graph of the comparison between the number of detected (orange boxes) and expected (gray triangles) proteins in each group. Twenty up-regulated protein classes and one down-regulated were identified.