Fig 1.
Crossing scheme adopted for the marker-assisted introgression of Xa38 in the genetic background of Improved Samba Mahsuri (ISM).
Fig 2.
Molecular detection of Xa38, Xa21, xa13 and xa5 in the BC3F2 plants through PCR using gene-linked markers Os04g53050-1, pTA248, xa13-Prom and 10603-T10Dw, respectively.
The marks shown on the top of the gel represent the positive BC3F2 plants for Xa38. Lane M: 100 bp molecular weight ladder; P1-Recurrent parent, Improved Samba Mahsuri; P2-Donor parent, PR 114 (Xa38).
Fig 3.
Analysis of genome introgression associated with the BB-resistant gene Xa38 on chromosome 4 in the eighteen selected BDLs of Improved Samba Mahsuri.
A genomic region limited to ∼0.6 Mb has been only introgressed from the donor parent PR 114 (Xa38) in the selected BDLs. The position of each polymorphic SSR marker in Mb on Chr. 4 is given adjacent to each marker.
Fig 4.
Reaction of the selected BDLs at the BC3F6 generation against bacterial blight of rice.
Forty-day-old plants were clip inoculated using a 3-day-old culture of IX-220 Xoo strain, and the lesion lengths (cm) were measured after 15 days of inoculation.
Table 1.
Phenotypic reaction of backcross-derived lines, parents and checks against Xoo strains.
Fig 5.
Panicles of selected BDLs at the BC3F6 generation.
The backcross-derived lines GLY # 78, 169, 209, 221, 302 and 327 possessed compact panicles with grain type similar to that of Improved Samba Mahsuri.
Table 2.
Agro-morphological characters of backcross-derived lines of Improved Samba Mahsuri.
Table 3.
Grain quality characteristics of parents and backcross breeding lines of ISM.