Fig 1.
Structure of thrombin binding aptamer (A) and novel isoguanine derivative of UNA (B).
Fig 2.
Chemical synthesis of UNA-isoguanine phosphoramidite.
Reagents: (i) LiNO2, AcOH, H2O; (ii) N,N-dimethylformamide dimethyl acetal, DMF; (iii) DMTCl, Py; (iv) NaIO4, 1,4-dioxane/water; NaBH4, 1,4-dioxane/water; (v) BzCl, DCM, -70°C; (vi) 2-cyanoethyl-N,N,N′,N′-tetraisopropylphosphordiamidite, tetrazole, MeCN.
Table 1.
Thermodynamic stability of TBA variants modified with RNA-iG (iGR) or UNA-iG (iGU)a.
Table 2.
Thermodynamic stability of TBA variants modified with RNA-iG and RNA-s4U (iGR,s4UR) or UNA-iG and UNA-s4U (iGU,s4UU)a.
Fig 3.
Representative circular dichroism spectra of TBA (black, solid line), and TBA variants substituted by RNA-iG (red, solid line), UNA-iG (blue, solid line), RNA-iG and RNA-s4U (red, dashed line), UNA-iG and UNA-s4U (blue, dashed line).
Fig 4.
Representative thermal difference spectra of TBA (black, solid line), and TBA variants substituted by RNA-iG (red, solid line), UNA-iG (blue, solid line), RNA-iG and RNA-s4U (red, dashed line), UNA-iG and UNA-s4U (blue, dashed line).
Fig 5.
Anticoagulant activity of TBA variants modified with RNA-iG or UNA-iG (A), RNA-iG and RNA-s4U (B), UNA-iG and UNA-s4U (C).