Fig 1.
PCoA graph using anthracnose score data based on whole plant assay of 11 isolates of C. gloeosporioides on different D. alata varieties.
The principle coordinate analysis (PCoA) graph indicates the virulence of 11 isolates when tested on different D. alata varieties based on detached leaf assay. Red: 11 C. gloeosporioides isolates; Blue: D. alata genotypes.
Table 1.
Characterization of C. gloeosporioides isolates based on anthracnose virulence of the isolates on different yam varieties for detached leaf technique (DLA) and whole plant assay (WPA).
Fig 2.
Characterization of C. gloeosporioides isolate Kog01R1 for virulence on two mapping population parents using detached leaf technique and whole plant inoculation.
Phenotypic evaluation of two parents based on detached leaf assay in the lab and whole plant assay in the screenhouse. 2A and 2B: detached leaf assay on TDa 95/00328 and TDa 95–310 in the laboratory; 2C and 2D: whole plant assay on TDa 95/00328 and TDa 95–310 in the screenhouse.
Fig 3.
Mean phenotypic distribution of 94 progenies for anthracnose disease for individual years and average across three years.
Frequency distribution of anthracnose disease among 94 progenies screened during 2011 (3a), 2012 (3b), 2013 (3c) and average across three years (3d). The arrows indicate the disease severity of the parental lines, TDa 95/00328 (Blue) and TDa 95–310 (Green).
Fig 4.
Genetic linkage map of water yam constructed using 380 EST-SSRs.
Distribution of EST-SSRs across 20 linkage groups.
Table 2.
Length, number of markers, and median distance between markers for each linkage group.
Table 3.
Summary of QTL analysis with significant QTLs identified in different years as well as the average over three years.
Fig 5.
QTLs for anthracnose disease based on average score across three years and SSR data at threshold LOD score using simple interval mapping (SIM).
Distribution of QTL associated with anthracnose disease. The bar represents the threshold LOD confidence for the QTL. QTL effects: Additive (Blue: Parent 1, Yellow: Parent 2); Dominance (Blue: Negative, Yellow-Red: Positive).