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Fig 1.

Landmark detection using multi planar reconstruction (MPR) with axial view as the centre of orientation.

Plotted landmarks: 1. Glabella, 2. Soft Nasion, 3. Hard Nasion, 4. Pronasale 5. Subnasale 6. Anterior nasal spine, 7. Sella 8 & 9. Alare 10 & 11. Orbitale 12 & 13 Porion 14 & 15 Zygion.

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Fig 1 Expand

Fig 2.

(I) Absolute error: AVEDEV and ODEV averaged for each landmark across all 20 individuals; soft-tissue landmarks are emphasized using a grey background: a) mean AVEDEV shown with a solid black line with its 2.5th-97.5th percentiles shown using broken grey lines; b) mean ODEV: solid line, operator 1; broken line, operator 2; dotted line, operator 3.

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Fig 2 Expand

Table 1.

Reproducibility (analysis II) of size and shape in the 20 women sample with three replicas: Procrustes ANOVA comparing individual variation, in centroid size and shape, to measurement error.

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Table 1 Expand

Fig 3.

(II) Reproducibility of centroid size visualized using jitter plots for the three sets of landmarks (nose, bone and all landmarks) using estimates from the three operators.

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Fig 3 Expand

Fig 4.

(II) Reproducibility of size: Scatterplot of nasal size used as an example of the graphical exploration of similarities across different operators: Operators 1 and 2 are shown respectively on the horizontal and vertical axes, while the size of the circles is proportional to size estimated from operator 3.

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Fig 4 Expand

Table 2.

Reproducibility (analysis II) of size and shape in the replica sample: Between operators pairwise correlations of centroid size (Pearson correlation) and shape (correlation of shape Procrustes distance matrices).

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Table 2 Expand

Fig 5.

(II) Reproducibility of shape: UPGMA phenogram based on Procrustes shape distances for the 20 women (each indentified by a progressive number from 1 to 20) digitized by all three operators: With high reproducibility, all three replicas, or at least two of them, should cluster together 'within' individual (black numbers); when this does not happen, numbers are shown using light grey.

As in Fig 4, nasal data are used as an example.

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Fig 5 Expand

Table 3.

Inter-operator bias (analyses II-III): R2 of centroid size and shape estimated in ANOVAs using operator as a grouping factor in the replica sample and the study sample.

For the study sample, R2 for sex, as main factor above operator, is also shown.

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Table 3 Expand

Fig 6.

(III) Inter-operator bias: Scatterplots of the first two PCs (principal components) of total shape (all 15 landmarks) accounting for respectively 15.5% and 11.0% of total variance; sex (a) and operator (b) are shown using different symbols. Despite PCs being computed regardless of a priori groups, operators (a meaningless grouping factor in the absence of bias) show less overlap than sexes (i.e., biological groups).

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Fig 6 Expand