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Fig 1.

Breeding population trends of four seabird species at Amsterdam Island since 1981.

Changes in (a) standardized population sizes and (b) breeding success.

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Fig 2.

Spatial locations of the individual seabirds which tested positive (red triangles) or negative (black dots) for (a) Pasteurella multocida and (b) Erysipelothrix rhusiopathiae on Amsterdam Island.

Horizontal axes represent longitude (x-axis) and latitude (z-axis); the vertical axis (y-axis) is the elevation of the nests above sea level. The Amsterdam albatross and the brown skua breed on the Plateau des Tourbières, while the Indian yellow-nosed and sooty albatrosses and the northern rockhopper penguin breed in the Entrecasteaux cliffs.

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Table 1.

Results of PCR detection of Pasteurella multocida and Erysipelothrix rhusiopathiae in biological samples collected between November 2011 and January 2012, from five seabird species breeding on Amsterdam Island.

CS: cloacal swabs, OS: oro-pharyngeal swabs, and RBC: red blood cell samples. Values are percentages of positive samples (95% confidence intervals).

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Table 2.

Pasteurella multocida positive cultures obtained from heart, lung, bone marrow and liver from 11 seabird carcasses.

All culture plates harbored a single, identical morphospecies, except for those two indicated by an asterisk.

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Fig 3.

Minimum-spanning tree of Pasteurella multocida sequence types (STs) based on the MLST Scheme (http://www.pubmlst.org/pmultocida/).

Each circle represents a ST (reference numbers are inside circles). ST61 is specified by a vertical black arrow. The color code indicates the animal origin of sequenced isolates available in the database, n represents the number of these isolates for each animal group.

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