Fig 1.
(A-D): Histological structure of preputial gland of Millardia meltada: ED-Excretory duct; E-Epidermis; SG-Sebaceous gland; Red arrow-the branched large sebaceous glandular lobules directly open into the skin surface; AG-Apocrine gland is a simple tubular gland and the secretion are found in the lumen (LU); NAHGL-Normal alveolar holocrine glandular lobules; MSGL-Modified sebaceous glandular lobules; SA-Serous acini; MA-Mucus acini; LD-Lipid droplets.
Fig 2.
(A-D): Histochemical features of preputial gland of Millardia meltada: S-sebum, LU-lumen.
Fig 3.
GC-MS chromatogram of crude extract of preputial gland of Millardia meltada.
The fractions are Undecane, Dodecane, Tridecane, Decane, Tetradecane, 1-chloro-octadecane, 6-methyl-1-heptanol, (ethenyloxy)-isooctane, 1,3-bis(1,1-dimethylethyl)-benzene, Pentadecane, 1-(3,4-dimethylphenyl)-ethanone, Heptadecane, Farnesol, 4,6-dimethyl dodecane, 1,1’-oxybis-octane, 1-dodecanol, 3,4-dimethyl-heptane, Pentadecane, 3,5-bis(1,1-dimethylethyl)-phenol, and 1-iodo-decane.
Table 1.
List of compounds identified in the crude extract of preputial gland of Millardia meltada.
Fig 4.
Protein profile in the representative 2-Dimensional electrophoresis of protein extract of preputial gland of Millardia meltada.
Fig 5.
Mass spectrum by LC-MS.
Fig 6.
Detection of protein concentration in purified fraction collection.
Fig 7.
SDS-PAGE of purified protein fraction.
Lane 1: Marker; Lanes 2–9: Purified protein (19kDa) from the crude extract.
Fig 8.
GC-MS identification of bound ligands as in the purified fractions 6-methyl-1-heptanol, and farnesol.
Table 2.
Bound form of volatiles in the purified fraction of preputial gland extract of Millardia meltada.
Fig 9.
(A-D): Immunohistochemical features of preputial gland of Millardia meltada P-primordial acini, S-sebum, F-fat substances, LU-lumen, α2uA-Alpha 2u-globulin antibody.