Fig 1.
Transposon mutagenesis of R. parkeri.
(A) Map of the pMW1650 plasmid used in this study for transposon mutagenesis (IR, inverted repeats). (B) Experimental scheme for transposon mutagenesis and isolation of individual mutants.
Fig 2.
Mapping the transposon insertion sites.
(A) Diagram showing the insertion of the transposon cassette into a chromosomal region (in grey). Primers specific to the transposon ends were paired with universal primers to amplify the chromosome- transposon junctions (red triangles), using semi-random nested PCR. Two nested PCR reactions were done to improve amplification of the chromosome-transposon junction directly from boiled bacteria. (B) R. parkeri chromosomal map showing all transposon insertion sites (see red lines) identified in this screen.
Table 1.
Transposon insertion sites in R. parkeri.