Fig 1.
SCN4B is downregulated in PTC tissues compared with normal thyroid tissues.
A. Heatmap of the expression of sodium channel subunits between PTC and 7 paired normal samples. Results were generated by reanalysis of the raw data of GSE3678. B-C. Heatmap (B) and plots chart (C) of SCN4B expression in PTC (N = 505) and normal thyroid tissues (N = 59). Data was obtained from TCGA-THCA. D-E. Representative SCN4B IHC staining (brown) in normal thyroid tissues (D) and in PTC tissues (E). Image credit: Human Protein Atlas. SCN4B images were obtained from: v18.proteinatlas.org, http://www.proteinatlas.org/ENSG00000177098-SCN4B/tissue/thyroid+gland#img and http://www.proteinatlas.org/ENSG00000177098-SCN4B/pathology/tissue/thyroid+cancer#ihc.
Fig 2.
Decreased SCN4B expression was associated with recurrence in classical PTC.
A-B. Plots chart of SCN4B expression between the PTC cases with or without metastasis (A) and between the cases with or without lymph nodal invasion (B). C. Heatmap showing the correlation between tumor recurrence and SCN4B expression in different PTC histological types. D. Plots chart of SCN4B expression between the classical/usual PTC cases with or without recurrence. Reanalysis was performed by using data from TCGA-THCA.
Fig 3.
Kaplan-Meier curves of RFS in patients with classical PTC.
Table 1.
The association between SCN4B expression and the clinicopathological parameters in patients with classical PTC.
Table 2.
Univariate and multivariate analysis of RFS in patients with classical PTC (SCN4B expression as categorical variables).
Table 3.
Univariate and multivariate analysis of RFS in patients with classical PTC (SCN4B expression as a continuous variable).
Fig 4.
DNA hypomethylation might be a mechanism of decreased SCN4B expression in PTC.
A. Heatmap showing the correlation between SCN4B expression and its DNA methylation (Methylation 450k) in different subtypes of PTC. B-C. Regression analysis of the correlation between SCN4B expression and its DNA methylation in all PTCs (B) and classical subtypes (C).