Table 1.
Subjects characteristics.
Fig 1.
Anti-Cut4 and anti-CFP21 antibody circulating secreting cells (ASCs).
(A) Anti-Cut4 and anti-CFP21 ASCs from representative subjects of the three TB groups using ELISpot assay. 0.1 million enriched B cells were seeded per well. Quantification of cells secreting spontaneously anti-Cut4 IgG (B) and anti-CFP21 IgG (C) in Mtb uninfected subjects and subjects with LTB and active TB. The median values have been drawn as horizontal lines. Values of Wilcoxon Mann Withney tests are indicated.
Fig 2.
Anti-Cut4 and anti-CFP21 memory B cells.
(A) Anti-Cut4 and anti-CFP21 ASCs after polyclonal stimulation from representative subjects of the Mtb uninfected and LTB groups, 1.105 enriched B cells were seeded per well. Quantification of ASC after five days of culture, anti-Cut4 IgG (B) and anti-CFP21 IgG (C) in Mtb uninfected and LTB subjects. The median values have been drawn as horizontal lines. Values of Wilcoxon Mann Withney tests are indicated.
Fig 3.
Serum antibody (IgG) levels against CFP21, Cut4 and PPD.
The dot plots indicate level of antibodies directed against Cut4 (A), CFP21 (B), and PPD (C) in Mtb uninfected subjects, LTB subjects, and TB subjects. Antibodies levels are indicated per index of the optical density (OD), corresponding to the ratio of sample OD450nm/blank OD450nm.