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Table 1.

Detection of carbapenemase alleles in clinical MDR-GNB isolates.

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Table 2.

Clinical characteristics, prior antibiotic usage, and underlying conditions in 119 patients colonized or infected with carbapenem-resistant MDR-GNB.

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Fig 1.

Distribution of MDR-GNB per hospital section.

Panel A: Departments, in which carbapenem-resistant MDR-GNB were isolated. Each affected patient was counted once per department. Panel B: Services that primarily cared for these patients. Each patient was counted once per service. A & E, accident and emergency; *excluding stem cell transplantation ward; **including hepatobiliary surgery.

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Fig 2.

Examples of strain typing results using pulse-field gel electrophoresis.

Panels A and B: Identical PFGE patterns generated from carbapenem-resistant MDR Acinetobacter baumannii (five individual patients A1-A5, panel A) and Klebsiella pneumoniae (five individual patients K1-K5, panel B) highly suggestive of nosocomial transmission. Panel C: Identical PFGE patterns of carbapenem-susceptible (P1) and carbapenem-resistant (P2) MDR Pseudomonas aeruginosa isolates from the same patient (blood culture and bronchioalveolar lavage, respectively) exemplifying acquisition of carbapenem-resistance under therapy. The time span between detection of the carbapenem-susceptible and carbapenem-resistant isolate was 21 days. Abbreviations: M, DNA Marker; C, unrelated clinical control isolate (same species, obtained outside the study period).

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Fig 3.

Colistin minimal inhibitory concentrations (MICs) for a) Klebsiella pneumoniae, b) Pseudomonas aeruginosa, and c) Acinetobacter baumannii isolates. Shown are non-duplicate isolates from individual patients. Dashed line: Clinical breakpoint according to EUCAST Breakpoint Table v.7.0 (MIC ≤ 2 mg/L, susceptible; MIC > 2 mg/L resistant).

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Table 3.

Distribution, initiation of treatment and in-hospital mortality of patients colonized or infected with carbapenem-resistant MDR-GNB.

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Table 3 Expand

Table 4.

Clinical characteristics, microbiology data and outcome of patients treated with ceftolozane/tazobactam or ceftazidime/avibactam during the study period.

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