Table 1.
Adsorption effect of Fe3O4@Au-C225 to antibody Fc.
Fig 1.
Transmission electron microscopy image of Fe3O4@Au composite MNPs (A); scanning electron microscopy image of Fe3O4@Au composite MNPs (B); The average diameter of Fe3O4@Au-C225 composite targeted MNPs (C); The zeta potential of Fe3O4@Au-C225 composite targeted MNPs (D); The UV–vis absorption spectra of Fe3O4@Au-C225 composite targeted MNPs (curve b) and Fe3O4@Au composite MNPs (curve a)(E); The hysteresis loops of Fe3O4@Au-C225 composite targeted MNPSs(F).
Fig 2.
The U251 cells labeled by Fe3O4@Au-C225 composite targeted MNPs at the concentration of 0.01mg/mL, 0.05mg/mL and 0.1mg/mL (A); The MRI of the U251 cells labeled by Fe3O4@Au-C225 composite targeted MNPs (a), Fe3O4@Au composite MNPs (b), both Fe3O4@Au-C225 composite targeted MNPs and C225 (c)(B); The T2WI relaxation time of the U251 cells labeled by Fe3O4@Au-C225 composite targeted MNPs (a), Fe3O4@Au composite MNPs(b), both Fe3O4@Au-C225 composite targeted MNPs and C225(c)(*compared with the control group, P<0.05)(C).
Fig 3.
The MRI T2*WI of the human transplanted glioma in mice labeled by Fe3O4@Au-C225 composite targeted MNPs(a), Fe3O4@Au composite MNPs(b), both Fe3O4@Au-C225 composite targeted MNPs and C225(c) (circle for the glioma)(A); The T2*WI relaxation time of the human glioma transplanted in nude mice labeled by Fe3O4@Au-C225 composite targeted MNPs(a), Fe3O4@Au composite MNPs(b), both Fe3O4@Au-C225 composite targeted MNPs and C225(c) at different time points (*compared with the 0h time point, P<0.05)(B).
Fig 4.
Schematic illustration of McAb-C225 blocking EGFR (A); Schematic illustration of C225 McAb adsorbed by Fe3O4@Au composite MNPs (B); Schematic illustration of McAb-C225 mediated endocytosis for Fe3O4@Au-C225 composite targeted MNPs (C).