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Table 1.

Demographic and clinical characteristics of patients carrying the 591, 106, 002 and other ribotypes.

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Fig 1.

Multiple correspondence analyses.

Variable categories contributing to dimension 1 (x-axis) and dimension 2 (y axis); variables with major contribution are close to the cero line in the chart. Ten variables contributing to both dimensions are shown.

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Fig 2.

Analysis of ribotype 591 by MLVA.

MLVA was applied to 27 isolates belonging to the ribotype 591. Dendrogram showing similarities of banding patterns generated with high-resolution capillary gel-based electrophoresis (CE)-PCR. MLVA clusters are identified by minimum-spanning tree: one clonal complex is defined by a MLVA type with less than a two-locus difference. In addition, free fecal toxin A/B (FFT), toxin in cell culture (TC), presence of PaLoc genes (tcdA/tcdB), minimal inhibitory concentrations (MIC) for vancomycin (VAN), metronidazole (MTZ), moxifloxacin (MOX) and clindamycin (CLIN) are shown for each isolate with the respective breakpoints. VNTR, variable-number tandem repeat.

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Fig 2 Expand

Fig 3.

Analysis of ribotype 106 by MLVA.

MLVA was applied to 12 isolates belonging to the ribotype 106. Dendrogram showing similarities of banding patterns generated with high-resolution capillary gel-based electrophoresis (CE)-PCR. MLVA clusters are identified by minimum-spanning tree: one clonal complex is defined by MLVA type with less than a two-locus difference. In addition, free fecal toxin A/B (FFT), toxin in cell culture (TC), presence of PaLoc genes (tcdA/tcdB), minimal inhibitory concentrations (MIC) for vancomycin (VAN), metronidazole (MTZ), moxifloxacin (MOX) and clindamycin (CLIN) are shown for each isolate with the respective breakpoints. VNTR, variable-number tandem repeat.

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Fig 3 Expand

Fig 4.

Analysis of ribotype 002 by MLVA.

MLVA was applied to 11 isolates belonging to the ribotype 002. Dendrogram showing similarities of banding patterns generated with high-resolution capillary gel-based electrophoresis (CE)-PCR. MLVA clusters are identified by minimum-spanning tree: one clonal complex is defined by MLVA type with less than a two-locus difference. In addition, free fecal toxin A/B (FFT), toxin in cell culture (TC), presence of PaLoc genes (tcdA/tcdB), minimal inhibitory concentrations (MIC) for vancomycin (VAN), metronidazole (MTZ), moxifloxacin (MOX) and clindamycin (CLIN) are shown for each isolate with the respective breakpoints. VNTR, variable-number tandem repeat.

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Fig 4 Expand

Table 2.

Antibiotic resistance profile of C. difficile strains of ribotypes 591, 106 and 002.

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Table 2 Expand