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Table 1.

Abundance of Wolbachia-like sequence reads in HTS from endemic New Zealand Orthoptera.

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Table 2.

Summary of New Zealand invertebrate samples tested for Wolbachia infection through PCR.

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Table 3.

Published representative Wolbachia genome diversity by host taxon.

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Fig 1.

The diversity of Wolbachia infections detected in New Zealand illustrated in a phylogeny of novel and published Wolbachia DNA sequences at the ftsZ locus (211–438 bp).

Species names are those of the hosts. Wolbachia supergroup A is in blue, and supergroup B in red. H.b Hemiandrus brucei, H.l Hemiandrus luna, H.m Hemiandrus maculifrons, H.n Hemiandrus nox.

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Fig 2.

Bayesian phylogenetic analysis of New Zealand and representatives of published Wolbachia endosymbionts based on ftsZ sequences.

Species names are those of the host of Wolbachia. Wolbachia supergroups are indicated by the corresponding letter (A–F). Host names in color (blue/red) are insect species endemic to New Zealand.

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Fig 3.

Evolutionary relationships among ftsZ DNA sequences from Wolbachia infections of New Zealand insects inferred using minimum spanning networks.

Published sequences from similar Wolbachia collected outside of New Zealand are coded in red. A. Supergroup (isolate) A Wolbachia (360 bp) infecting New Zealand orthoptera and parasitoid wasps. B. Supergroup (isolate) B Wolbachia (228 bp) infecting orthoptera and book lice. Numbers of nucleotide differences among FtsZ sequences are indicated.

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Fig 4.

Location and Wolbachia infection status of two native orthopteran lineages in New Zealand.

A. Collection locations of Hemiandrus individuals indicating Wolbachia isolate found; Isolate A blue, Isolate B orange, infected but not sequenced white, not infected black, populations containing both Isolate A and Isolate B red. B. Collection locations of Rhaphidophoridae (cave weta) indicating Wolbachia infection status; infected white, not infected black. Inset images, female Hemiandrus brucei (left) and Isoplectron armatum (right).

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