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Fig 1.

Categorization of districts in the Republic of Serbia according to risk of WNV outbreak.

The figure show the geographic position of Republic of Serbia in Europe. The districts (NUTS3) with higher risk of WNV infection are marked in red (10 districts in WNV surveillance programme in 2014 and 11 districts in WNV surveillance programme in 2015), and the districts with lower risk of WNV infection are marked in yellow. The solid black lines in the maps of Republic of Serbia represents the borders of districts (25 districts in Serbia that were under WNV surveillance in 2014 and 2015). The northernmost seven districts represent the Autonomous Province of Vojvodina. Description of the sampling locations: Districts names, geographic coordinates and web links are provided as the supporting information S3 Table. The basic administrative maps were extracted from the GADM database of Global Administrative Areas (www.gadm.org), version 2.8, November 2015, and later on changed according to the data presented in them.

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Table 1.

WNV surveillance plan (sampling and testing) in 2014.

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Table 2.

WNV surveillance plan (sampling and testing) in 2015.

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Fig 2.

Distribution of WNV activity detected during the 2014 surveillance programme at the district level.

Figs 2a-d show WNV infection rates as percentages (number positive samples/number of samples tested) for a) sentinel chickens b) sentinel horses, c) mosquito pools and d) wild birds. The basic administrative maps were extracted from the GADM database of Global Administrative Areas (www.gadm.org), version 2.8, November 2015, and later on changed according to the data presented in them.

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Fig 3.

Distribution of human West Nile cases reported during the 2014 at the district level.

Fig 3 shows the number of clinical and laboratory confirmed human West Nile cases reported to the ECDC (except for one clinical case in Raška and Nišava district that were not laboratory confirmed). The basic administrative maps were extracted from the GADM database of Global Administrative Areas (www.gadm.org), version 2.8, November 2015, and later on changed according to the data presented in them.

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Fig 4.

The temporal distribution of the first positive results detected during WNV surveillance and the time of the first human West Nile cases in 2014.

The data for the first positive results obtained during WNV surveillance and the first reported human cases in 2014 are presented by symbols per district level. The top seven districts represent Vojvodina Province, followed by the other districts of Serbia sorted in alphabetical order.

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Fig 5.

Distribution of WNV activity detected during the 2015 surveillance programme at the district level.

Fig 5a-c show WNV infection rates as percentages (number positive samples/number of samples tested) for a) wild birds b) horses, and c) mosquito pools. Fig 5D presents the number of clinical and laboratory confirmed human West Nile cases reported to the ECDC. The basic administrative maps were extracted from the GADM database of Global Administrative Areas (www.gadm.org), version 2.8, November 2015, and later on changed according to the data presented in them.

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Fig 6.

The temporal distribution of the first positive results detected during WNV surveillance and the time of the first West Nile human cases in 2015.

The first positive results obtained during WNV surveillance and the first reported human West Nile cases in 2015 are represented by symbols per district level. The top seven districts represent the Vojvodina Province, followed by the other districts of Serbia sorted in alphabetical order.

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