Fig 1.
A. The 5’ upstream promoter sequences of the CsPSY gene. Some important cis-regulatory elements present in CsPSYp are highlighted in different color. Putative TATA box and transcription start sites are shown in red color. 1B. The 5’ upstream promoter sequences of the CsUGT gene. Some important cis-regulatory elements present in CsUGTp are highlighted in different color. Putative TATA box and transcription start sites are shown in red color.
Table 1.
Predicted cis-regulatory elements in CsPSY promoter.
Table 2.
Predicted cis-regulatory elements in CsUGT promoter.
Fig 2.
Histochemical analysis of GUS activity.
(Up)—For qualitative analysis, infiltrated tobacco leaves (with respective construct) and (Down) transformed Crocus calli (with respective construct) were separately subjected to histochemical GUS staining.
Fig 3.
Effect of ABA and GA on CsPSY and CsUGT promoter activity.
For qualitative analysis, ABA and GA treated (Up) CsPSYp (Down) CsUGTp transformed Crocus calli were separately subjected to histochemical GUS staining.
Fig 4.
Relative expression of CsPSY and CsUGT after ABA and GA treatment.
qRT-PCR analysis of CsPSY and CsUGT transcripts in ABA and GA treated flowers and leaves of C.sativus at 12 and 24 hrs post treatment.
Fig 5.
Relative expression of TFs preferably expressing in stigma.
qRT-PCR analysis of CsbHLH, CsWRKY, CsAUX, CsSNF, CsARF and CsMYB transcripts in ABA and GA treated flowers of C.sativus at 12 and 24 hrs post treatment.
Fig 6.
Probable mechanism of regulation.
Picture showing probable regulatory mechanism of CsPSY and CsUGT promoter activity.