Table 1.
Classification of the species corresponding to the TCS-CM genus Colletopterum Bourguignat, 1880.
Fig 1.
Map of the study area with anodontine specimens sampling sites (red circles).
A–location of Lake Baikal in Russia territory, B–Map of Lake Baikal and Transbaikalia region; Lake Baikal: 1 –Cherkalov Sor and 2 –Chyvyrkuy Bay; Baikal Basin (3 –River Selenga, 4 –Lake Torma, 5 –Lake Schuchje, 6 –Gusinoye, 7 –River Khilok); River Lena basin (8 –Lake Bol’shoe Eravnoe); Baikal-Lena basin (9 –Ivan-Arachley lake system including Lakes Shaksha, Arachley, Kergendu, Ivan and Tasey), closed lake-refuges (10 –Lake Kenon, 11 –Lake Arey).
Fig 2.
Shell measurements used for morphometry and species identification.
1 –lateral shell view showing: the distance from umbo apex to anterior shell margin (l) and shell maximal height (Hm) from umbo apex to ventral shell margin across maximal convex point of valves (mcp); 2 –dorsal shell view showing the mcp; 3 –anterior shell view and shell width (B) in the maximal convex point (mcp); 4 –arcs of maximal convexity of the valve outline of Baikalian species (Prozorova & Bogatov, 2006): A–C. ponderosum Pfeiffer 1825, B–C. anatinum Linnaeus 1758, C–C. piscinale Nilsson 1822, D–C. nilssonii Küester 1842, E–C. sorensianum Dybowsky, 1913; 5 –arcs of maximal convexity of the valve outline of Eurasian species: g– С. rostratum Rossmaessler 1836, h–C. ponderosum, i–C. anatinum, j– С. piscinale, k–C. nilssonii, l–C. sorensianum, and 6 –of European species: a–C. apollonicum (Bourguignat 1880), b–C. convexum (Drouet 1888), c–C. milaschevichi Bogatov, Starobogatov et Prozorova 2005, d–C. ostiarium (Drouet 1881), e–C. baeri Bogatov, Starobogatov et Prozorova 2005, f–C. subcirculare (Clessin 1873) according to Bogatov et al. (2005).
Table 2.
List of all individual specimens used for genetic analyses, collection sites and GenBank accession codes.
Table 3.
Shell metrics and TCS-CM identification of collected specimens.
Fig 3.
Umbo sculpture in Colletopterum species from studied region of Lake Baikal and Transbaikalia.
Fig 4.
Inner anatomy features in Colletopterum specimens from Lake Baikal and Transbaikalia.
General view of soft body 4.1 –male, 4.2–4.3 –female with mature glochidia: m–mantle, ig–inner gills, og–outer gills, lp–labial palps, f–foot, ia–inner aperture, ea–excurrent aperture, sa–supra-anal aperture, aam–anterior adductor muscle, pam–posterior adductor muscle; 4.4: pia1 –retracted position and pia–normal position of papillae in the inner aperture, 4.5–4.8: pia–papillae shape of the inner aperture and pg–different pigmentation of excurrent aperture, 4.9 –papillae scaled-up.
Table 4.
TCS-CM species distribution of collected specimens.
Presence +, Absence -.
Fig 5.
Intraspecific variability and dynamic of mean values of morphometric shell indexes.
B/H– 5.1, H/L– 5.2, and l/L– 5.3 in Colletopterum species, range and change of mean values of B/H– 5.4 and shell length– 5.5 with increase of animal age from different localities in Lake Baikal, Transbaikalia and European Russia.
Fig 6.
Change of shell shape and shell convexity with growth of shell length and index B/H in different age groups of TCS-CM Colletopterum species from different localities.
B–Lake Baikal, T–Transbaikalia, ER–European Russia: 1 –young and group of C. sorensianum including C. subcirculare and C. baeri, 2 –group of C. nilssonii including C. ostiarium and C. milaschevichi, 3 –group of C. piscinale including C. convexum, 4 –group of C. anatinum with transitional form C. apollonicum, 5 –group of C. ponderosum and 6 –group of С. rostratum.
Fig 7.
COI haplotype (TCS) network showing the relationships A. anatina haplotypes.
Colour codes correspond to the 5 haplogroups: Blue—Northwest Iberia; Green—Southcentral Iberia; Red Southwest Iberia; Purple—Europe; Yellow—Western Mediterranean. Within the European haplogroup, Russian haplotypes are represented in dark purple and Ukrainian in light purple. Circle size is proportional to the observed haplotype frequencies, white dots represent unobserved haplotypes.