Fig 1.
Decision tree representing the conventional diagnostic workflow for PUO in adults based on a clinical guideline [1], and the subsequent introduction of metagenomic pathogen testing as first- or second-line investigations.
Table 1.
Investigations recommended for work-up of PUO in the clinical guideline [1] with modifications.
The total cost for each line of investigations are shown in Singapore dollars (SGD).
Table 2.
Probability of disease-specific diagnostic outcome (without next-generation sequencing).
Table 3.
List of parameters in break-even analysis.
Fig 2.
Expected cost of next-generation sequencing (NGS) strategies vs. current standard of care (baseline scenario).
Table 4.
Results of break-even analysis.