Table 1.
List of qPCR primers used in this study.
Fig 1.
Symptoms on leaves of MIMV-inoculated maize (A) compared with leaves of mock-inoculated plants (B), 15 days after exposure viruliferous (A) or non-viruliferous plant hoppers (B).
Table 2.
Raw data statistics of MIMV-infected maize and uninfected maize libraries.
Fig 2.
Volcano plot (t-test) showing MIMV-maize RNA-Seq data.
X-axis shows fold-change of gene expression and y-axis shows statistical significance (–log10 of the p-value). Down-regulated transcripts are plotted on the left, up-regulated transcripts on the right.
Fig 3.
Validation of MIMV responsive up-regulated maize genes.
RT-qPCR (Black bars) vs RNA-Seq (Grey bars) fold-change expression of selected genes in three biological replicates (1–3). PT: Protein transporter, HSPF: Heat shock protein factor 7, PP: Pathogenesis protein, TIF: Transcription initiation factor TFIID subunit 11, TBI: Transmembrane BAX inhibitor motif containing protein.
Table 3.
Top 13 up-regulated transcripts in MIMV-infected compared to uninfected maize.
Fig 4.
Gene ontology categories of up-regulated transcripts in maize leaves in response to MIMV infection determined using Blast2GO.
Fig 5.
Gene ontology categories of down-regulated transcripts in maize leaves in response to MIMV infection determined using Blast2GO.
Table 4.
Top 13 down-regulated transcripts in MIMV-infected compared to uninfected maize.
Fig 6.
KEGG pathways for up-regulated enzymes determined using BLAST2GO.
Black color shows the number of transcripts involved in each process and gray color shows the number of enzymes involved.