Fig 1.
Typical EEG pattern of spontaneous seizures (A) and schematic description of the parameters (B). (A) Typical EEG recording of seizure clusters (upper) and a spontaneous seizure (lower). Upper: The 2-hour EEG trace shows that seizures occurred in a cluster in the latter part of the trace. A seizure in the upper EEG trace (black box) is presented in the lower EEG trace. (B) a (both a’ and a”): seizure cluster, a’: fully monitored (FM) cluster, a”: seizure cluster that was not fully monitored, b: seizure outside a seizure cluster, c: cluster interval, d: cluster duration, e: seizure-free period, f: peak seizure frequency.
Fig 2.
Daily frequency of spontaneous recurrent seizures in all individual mice.
The seizure frequencies (spontaneous recurrent seizures/day) in individual mice are represented by the gray bar: light color indicates a low seizure frequency, and dark color indicates a high seizure frequency. The start and end of the monitoring period are marked by the vertical dotted lines. (A) Chronic phase 1. (B) Chronic phase 2. SE = status epilepticus, SRS = spontaneous recurrent seizure.
Fig 3.
Video-EEG monitoring data from a representative animal.
Video-EEG monitoring data from a representative animal. (A) The number of spontaneous seizures per day. (B) Raster plot of the seizure frequency on the day of peak seizure frequency. The data are extracted from animal #4 in the chronic phase 2 group.
Fig 4.
Scatter plots of various parameters.
Scatter plots of cluster duration vs total number of spontaneous recurrent seizures during a single cluster (A), cluster duration vs duration of the following seizure-free period (B), number of days from cluster onset to the day of peak seizure frequency vs peak seizure frequency (C), and number of days from cluster onset to the day of peak seizure frequency vs number of days from the day of peak seizure frequency to cluster end (D). The dotted line represents a slope of 1.
Table 1.
Characteristics of spontaneous recurrent seizures in a mouse pilocarpine-induced epilepsy model in chronic phase.
Table 2.
Comparison of characteristics of spontaneous recurrent seizures with a previous study using our definition of seizure cluster.