Table 1.
HBV genetic diversity in three infected individuals characterized by sequencing whole genome PCR products using three different methods.
Table 2.
LAST read mapping statistics for HBV sequencing runs on the MinION™ device.
Table 3.
Read mapping statistics for HBV sequencing runs after error correction using NanoCorrect.
Fig 1.
Schematic representation of the recombinant HBV strain B5584.
The HBV genome is represented in a circular form and the HBV open reading frames are shown. Breakpoint positions for D (red, dotted)/E (blue, striped) genomic recombination are indicated with arrows and positions are given relative to an HBV genotype A reference sequence (GenBank AM282986).
Fig 2.
Phasing of the S gene mutations Thr116Asn and Thr118Ser in the HBV genome.
Each bar represents one read and the dots indicate a deletion. Both single nucleotide variants were called by MinION (A) and direct Sanger sequencing (B). The number of MinION reads called for each of the single nucleotide variant patterns (C).
Fig 3.
Alignment of nanopore reads of B6260.
Complete and partial Nanopore sequence reads were aligned with the corresponding Sanger consensus sequence used as reference. The viral core, surface, polymerase and X proteins are indicated and positions provided according to the reference sequence AM282986. Four different types of Minion reads are shown: nearly complete HBV genome (blue); reads showing a 123-nt deletion (positions 2,968–3,090) in the PreS1 region (purple); reads with the 123-nt and an additional 24-nt deletion (positions 501–524) in the “a determinant” of the S region (green); and unclassified partial reads (yellow).