Fig 1.
Establishment of patient-derived tumor spheroids over 120 days.
Representative bright-field images of the tumor spheroids were shown and taken at x 40 total magnifications. Scale bars correspond to 200 microns.
Fig 2.
Patient-derived tumor spheroids maintain the cytological features and markers of the primary tumors.
Representative images of the (a) H&E staining. scale bars correspond to 100 microns; (b) immunostaining of primary tumor, patient-derived tumor spheroid and/or H1299 tumor spheroid were shown. Scale bars correspond to 100 microns.
Fig 3.
Cytotoxicity of cisplatin in patient-derived tumor spheroids and H1299 tumor spheroids.
Cell viability was assessed using CellTiter-Glo 3D Cell Viability Assay after 72h of drug exposure. Data are mean ± S.D. obtained from one independent experiment done in triplicates.