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Fig 1.

SEM image of strain A6 in an acidic, iron reducing, and ammonia oxidizing culture (iFeN).

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Fig 2.

Phylogenetic tree derived from neighbour-joining analysis of partial 16S rRNA sequences.

The tree was constructed with 16S rRNA gene sequences from strain A6 (MG589453) and from other bacteria of the Acidimicrobiaceae family. Sequences determined in this study are in bold. Bootstrap values were based on 1000 replicates each and are shown at the nodes with >50% bootstrap support. The scale bar represents 1% sequence divergence.

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Fig 3.

Concentration of NH4+, NO2-, Fe(II), and Fe(III) over 20 days of incubation in the liquid iFeN media with Acidimicrobiaceae sp. A6.

The values represent the mean and standard error (n = 3).

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Fig 4.

Amount of NH4+ removal (black) and Fe(III) reduction (white) over 14 days of incubation with Acidimicrobiaceae sp. A6.

Under different (a) pH values, (b) iron source, (c) carbon source, and (d) AQDS concentions. The values represent the mean and standard error (n = 3).

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