Table 1.
Voucher sample information and GenBank accession numbers of sequence data used in this study.
Fig 1.
SEM images of ascospores for Asian black truffles, showing the details of ornamentation.
A–C: Tuber longispinosum (A: K70, B: K209, C: K466), D–F: Tuber sp. 6 (D: K152, E: S4, F: S23), G–I: T. formosanum (G: HKAS48268 paratype, H: HKAS62628 holotype, I: HKAS79547), J–L: T. himalayense (J: HP1-3, K: MY5-1, L: SHD1-1-1), M–O: T. indicum (M: YSH1-8, N: YR1-4, O: HD6-16). Bars = 10 μm.
Fig 2.
Ascospore spine height of four-spored asci (A) and width of spine bases (B), for the studied taxa. Different letters above boxes indicate significant differences between mean according to Tukey–Kramer honestly significant difference test (P < 0.01).
Fig 3.
Relative frequencies of asci with one to six ascospores per ascus for the studied taxa.
Fig 4.
Phylogenetic relationships among Asian black truffles based on MAT1-1-1 and MAT1-2-1 sequences.
The phylogram was obtained by maximum likelihood inference under the TN93 model. SH-aLRT values and Bayesian posterior probabilities are shown as ML/BPP.
Fig 5.
Phylogenetic relationships among Asian black truffles based on three combined datasets (ITS, β-tublin, and TEF1-α).
The phylogram was obtained by maximum likelihood inference under the TN93+I model. SH-aLRT values and Bayesian posterior probabilities are shown as ML/BPP.
Fig 6.
Tuber longispinosum photographs (holotype, TFM: S17009).
A. Dried ascomata (bar = 1 cm). B. Fruit bodies photographed in the field. C. Peridial warts, (bar = 3 mm). D. Asci and ascospores (bar = 30 μm). E. Ascospore (bar = 10 μm). F. Peridium in cross section (bar = 50 μm).
Fig 7.
Tuber himalayense photographs (TFM: S17015).
A. Ascomata (bar = 1 cm). B. Peridial warts, (bar = 3 mm). C. Asci and ascospores (bar = 30 μm). D. Peridium and glebal tissue in cross section (bar = 50 μm). E. Ascospore (bar = 10 μm).