Fig 1.
Antibody profiles for MuSK, LRP4, and clustered AChR in patients with MG seronegative for AChR antibody on radioimmunoprecipitation assay.
LEMS, Lambert-Eaton myasthenic syndrome; MND, motor neuron disease; MG, myasthenia gravis; MuSK, muscle-specific tyrosine kinase; CBA, cell-based assay; RIPA, radioimmunoprecipitation assay; LRP4, low-density lipoprotein receptor-related protein 4; cAChR, clustered acetylcholine receptor.
Fig 2.
Results of CBA for antibodies to MuSK, LRP4, and clustered AChR (A) and correlation between CBA scores and RIA values for anti-MuSK antibodies (B) MuSK, muscle-specific tyrosine kinase; CBA, cell-based assay; LRP4, low-density lipoprotein receptor-related protein 4; AChR, acetylcholine receptor; RIA, radioimmunoassay.
Fig 3.
Cell based assays to detect antibodies to clustered AChR, MuSK or LRP4.
Patient IgG binding is shown in red. For the AChR and MuSK assays, EGFP tags exhibit a green fluorescence highlighting the transfected cells; there is no color tag in the LRP4 assay. Patient 41 is positive for AChR, Patient 42 is positive for MuSK antibodies, and patient 72 is low positive for LRP4 antibodies. MuSK, muscle-specific tyrosine kinase; LRP4, low-density lipoprotein receptor-related protein 4; AChR, acetylcholine receptor.
Table 1.
Clinical features of AChR antibody seronegative generalized MG (on radioimmunoprecipitation assay) according to the antibody profile.
Table 2.
Clinical features of the patients with the LRP4 antibody, and of the patients who were double seropositive.