Fig 1.
Alveolar structure and schematic design of isotope tracing.
3H-labeled methyl chloride choline is involved in surfactant phospholipid synthesis of alveolar type II cells. The level of 3H in phospholipids was monitored to detect the amount originating from endogenous phospholipid synthesis. The radioactivity of TPL and DSPC in BALF and LT indicates the total content of 3H-labeled choline chloride incorporated into TPL and DSPC, which reflects the newly synthesized TPL and DSPC and the body’s ability to synthesize PC. The secretory rate of TPL was expressed as the ratio of radioactivity in BALF and the whole lung (BALF+LT). The secretory rates of TPL and DSPC indicate the ability of alveolar type II epithelial cells to secrete PC into the alveolar space.
Fig 2.
Histopathological characterization in rats as determined by gross morphology and hematoxylin-eosin (H-E) staining (×400).
A and D: Normal control group; B and E: OA-PE group; C and F: OA-PS treatment group. A, B, C: gross morphology; D, E, F: H-E staining.
Fig 3.
Electron microscopy observations of pulmonary surfactant layer (PSL) and vascular endothelial cells.
A and D: Normal control group, B and E: OA-PE group, C and F: OA-PS treatment group. A, B, C: PSL; D, E, F: vascular endothelial cells.
Table 1.
Lung injury scores (LIS) in the three experimental groups.
Table 2.
Changes in TPL and DSPC in lung tissue.
Table 3.
Changes in TPL and DSPC in BALF.
Fig 4.
Changes in 3H-TPL and 3H-DSPC levels in LT and BALF.
A: Changes in 3H-TPL and 3H-DSPC levels in LT. B: Changes in 3H-TPL and 3H-DSPC levels in BALF. C: Changes in TPL SR and DSPC SR.