Table 1.
Baseline demographic and clinical characteristics of study participants.
Table 2.
Evaluated immunoassay kits, including assay characteristics and CSF dilutions.
Fig 1.
Structure of the assay stability evaluation scheme.
Duplicate samples were located on individual plates as shown, to allow for assessment of intra- and inter-assay precision and biotemporal stability of analyte measures. T1 and T2 denote repeat-collected CSFs from the same individual; A-C indicate different aliquots of the same CSF sample. N = 35 samples were included on each individual plate, in addition to a standard curve and spiked controls.
Fig 2.
Short-term biotemporal stability of measurable and technically precise analytes in CSF.
Biotemporal variation between paired samples over an 8-week interval for (a) core AD biomarkers, (b) Aβ and tau-independent markers of neurodegeneration, (c) metabolic and oxidative stress markers, (d) markers of inflammation and inflammatory modulators, and (e) vascular injury markers. Samples plotted for each analyte, with baseline concentrations connected to corresponding paired week 8 concentrations. Plotted using a log scale, all units converted to pg/mL. N = 9 pairs for each analyte, concentrations averaged across three technical replicates. Each analyte plot is labeled with the median biotemporal CV (%). A CV < 15% indicated low intra-individual variation.
Table 3.
Intra-individual biostability of analyte measurements between repeat-collected CSFs.