Fig 1.
Morphology of the ACC interspecific hybrid between B. napus and B. oleracea and its microspore-derived lines.
(A) Zhongshuang 9 seedling; (B) 6m06 seedling; (C) hybrid ACC seedling; (D-I) Seedling of microspore-derived lines from the hybrid between Zhongshuang 9 and 6m08.
Fig 2.
Cytology of the ACC hybrid and its microspore-derived lines from B. napus and B. oleracea.
(A) One cell of ACC with chromosome number 28; (B) One PMC of ACC with 9II + 10I; (C) One PMC of ACC with 10II + 8I; (D) One PMC of ACC with 9:19; (E) One PMC of ACC with 10:18; (F) One PMC of ACC with chromosome bridge; (G) One microspore-derived line with 18 chromosomes; (H) One microspore-derived line with 38 chromosomes; (I) One microspore-derived line with 52 chromosomes. Those marked with stars were bivalent.
Table 1.
Comparing the genetic distance of B. napus-like individuals, aneuploid and unreduced gametes of microspore-derived lines to the parental species, natural B. napus and B. oleracea.
Fig 3.
Phylogenetic trees showing the relationships between 30 MD progeny (red lines) and 42 B. oleracea (blue lines), 34 B. napus (green lines).