Table 1.
Summary of clinicopathological parameters of the IHC cohort.
Table 2.
Characteristics of the patients included in the survival study–qPCR Cohort.
Fig 1.
Expression and correlation of HIF-1α, HIF-2α and HAF in ccRCC.
(A, C and E) Positive nuclear staining to HIF-1α, HIF-2α and HAF, respectively, in primary ccRCCs at high magnification (x100). (B, D and F) Heterogenous nuclear staining of HIF-1α, HIF-2α and HAF, respectively, in a tumor at low magnification (x20). (G, H and I) Correlation of HIF-1α, HIF-2α and HAF, respectively, with the Fuhrman grade.
Fig 2.
Expression and correlation of GLUT1 in ccRCC.
(A) Positive membranous staining to GLUT1 in primary ccRCCs at high magnification (x100). (B) Heterogenous membranous staining to GLUT1 in a tumor at low magnification (x20). (C) Correlation of GLUT1 with the Fuhrman grade.
Table 3.
Correlation between the expression score of each protein compared to the low-grade contingent (Fuhrman grade II) and the high-grade (Fuhrman grades III and IV) contingent and the characteristics of the immunochemistry using the Wilcoxon rank-sum test.
Table 4.
Correlation between the Fuhrman grade and clinicopathological parameters using the Kendall nonparametric rank test.
Table 5.
Correlation between pT stage and clinicopathological parameters using the nonparametric rank test of Kendall.
Table 6.
Correlation between the tumor diameter and the characteristics of the immunochemistry using the Kendall nonparametric rank test.
Fig 3.
Expression and correlation of MCT1 and MCT4 in ccRCC.
(A and C) Positive membranous staining to MCT1 and MCT4 in primary ccRCCs at high magnification (x100). (B and D) Heterogenous membranous staining to MCT1 and MCT4 in a tumor at low magnification (x20). (E and F) Correlation of MCT1 and MCT4, respectively, with the Fuhrman grade.
Fig 4.
Expression and correlation of CAIX and CAXII in ccRCC.
(A and C) Positive membranous staining to CAIX and CAXII in primary ccRCCs at high magnification (x100). (B and D) Heterogenous membranous staining to CAIX and CAXII in a tumor at low magnification (x20). (E and F) Correlation of CAIX and CAXII, respectively, with the Fuhrman grade.
Fig 5.
Overexpression of MCT4 and CAIX did not correlate with reduced overall survival of non-metastatic ccRCC patients.
Kaplan–Meier analysis of OS of M0 patients. OS was calculated from patient subgroups with mRNA levels that were less or greater than the third quartile. Statistical significance (p values) is indicated.
Fig 6.
Overexpression of GLUT1 and MCT1, but not CAXII, correlated with reduced overall survival of non-metastatic RCC patients.
Kaplan–Meier analysis of OS of M0 patients. OS was calculated from patient subgroups with mRNA levels that were less or greater than the third quartile. Statistical significance (p values) is indicated.