Table 1.
Primers and sequence information.
Fig 1.
C. elegans Pgps genes profile using 2-ΔΔCt method.
Pgps gene expression for WT strains are represented by dashed line. P values were obtained with One sample t test, comparing sample value to WT expression reference value.
Fig 2.
Evaluation of RNAi-mediated impairment of Pgp 12 expression in the WT and IVR30 strain.
(A) Percentage of L3 paralysis after 48 hours of ivermectin exposure for groups Pgp 12 silenced [RNAi+], Pgp 12 non silenced [RNAi-] and negative control [CT], cultured only in M9 medium and not exposed to ivermectin treatment. (B) Assessment L3 viability cell using propidium iodide marker after 48 hours ivermectin exposure in Pgp 12 silenced [RNAi+] and Pgp 12 non silenced [RNAi-] groups. P value refers to one-way ANOVA test with Turkey’s multiple comparisons test.
Fig 3.
Evaluation of RNAi-mediated impairment of Pgp 4 expression in the WT and IVR30 strain.
(A) Percentage of L3 paralysis after 48 hours of ivermectin exposure for groups Pgp 4 silenced [RNAi+], Pgp 4 non silenced [RNAi-] and negative control [CT], cultured only in M9 medium and not exposed to ivermectin treatment. (B) Assessment of L3 viability cell using propidium iodide marker after 48 hours ivermectin exposure in Pgp 4 silenced [RNAi+] and Pgp 4 non silenced [RNAi-] groups. P value refers to one-way ANOVA test with Turkey’s multiple comparisons test.
Fig 4.
Evaluation of RNAi-mediated impairment of Pgp 4 and 12 expression in the WT and IVR30 strain.
(A) Percentage of L3 paralysis percentage after 48 hours ivermectin exposure for groups Pgps 4 and 12 silenced [RNAi+], Pgps 4 and 12 non silenced [RNAi-] and negative control [CT], cultured only in M9 medium and not exposed to ivermectin treatment. (B) Assessement of L3 viability cell using propidium iodide marker after 48 hours ivermectin exposure in Pgps 4 and 12 silenced [RNAi+] and Pgps 4 and 12 non silenced [RNAi-] groups. P value refers to one-way ANOVA test with Turkey’s multiple comparisons test.
Fig 5.
In silico analysis revealing a close relationship between Pgps from C. elegans and nematodes parasites represented.
As follow: Ascaris suum (A), Brugia malayi (B), Cooperia oncophora (C), Haemonchus contortus (H), Loa loa (L), Onchocerca volvulus (O), Parascaris equorum (P), Strongyloides ratti (S), Trichuris trichiura (T), Wuchereria bancrofti (W), C. brenneri (1), C. briggsae (2), C. remanei (4), Pgp 4 of C. elegans (Δ), Pgp 12 of C. elegans (•), all remaining Pgps from C. elegans (2).