Fig 1.
STR loci (A) and karyotype of the MSC at 6th passage of cultivation: GTG (B) and mFISH (C) staining.
Table 1.
Clonal and non-clonal chromosome aberrations and genome variations at different MSC passages.
Table 2.
Frequency of chromosomal aberrations and breaks in MSC.
Fig 2.
Localization of chromosomal translocations breakpoints in MSC.
The width of the blue columns opposite the band corresponds to the number of breaks in this region.
Fig 3.
Clones with karyotypic abnormalities in MSC.
A. Karyogram of the MSC clone with tetrasomy 8 (multicolor FISH, clone 1). B. The nucleus of MSC with 4 centromeric signals of chromosome 8 (green) and 2 chromosomes 6 (orange) (tetrasomy 8). C. The nucleus of MSC with 4 centromeric signals of chromosome 8 (green) and 4-chromosome 6 (orange) (polyploidy). Clonal chromosome translocations: D—clone 2 with two balanced translocations (D1 and D2), E—clone 3 with loss of part of chromosome 6, F—clone 4 with one balanced translocation.
Table 3.
A FISH-analysis with centromere-specific DNA probes of the chromosomes 6 and 8 in interphase nuclei.
Fig 4.
Histogram showing the distribution of chromosome counts in MSC at the different passages.
Fig 5.
Schematic karyotype structure of MSC.