Table 1.
Summary of sequencing and genome data for the four Irish EHV-8 strains.
Fig 1.
Maximum likelihood phylogenetic tree of ORF30 amino acid sequences based on the JTT matrix-based model.
Details of the sequences are in S1 Table of the Supporting Information. The scale bar represents the number of substitutions per site. Bootstrap values after 1000 replications are indicated at major nodes.
Fig 2.
Map of the equid herpesvirus 8 (EHV-8) genome.
The coordinates are based on the 149600 bp sequence of EHV-8/IR/2003/19. Predicted protein-coding regions are shown by coloured arrows with names below. Red open reading frames (ORFs) were inherited from the ancestor of family Herpesviridae and blue ORFs from the ancestor of subfamily Alphaherpesvirinae. Orange ORFs are specific to certain lineages within subfamily Alphaherpesvirinae. Inverted repeats TRS/IRS flanking US are coloured yellow, and inverted repeats TRL/IRL flanking UL are shown by green vertical lines at 1–32 and 112999–113030 bp. A third green vertical line marks a third copy of the TRL/IRL sequence that is part of a more extensive inverted repeat at 1–87 and 547–633 bp. Tandem reiterations are marked by grey shading, and origins of DNA replication by vertical red lines.
Fig 3.
Conserved sequences near the genome termini of equid varicelloviruses.
An alignment of sequences from the four Irish equid herpesvirus (EHV) 8 (EHV-8) strains, EHV-9 P19 (AP010838.1), EHV-1 AB4 (AY665713.1) and EHV-4 NS80567 (AF030027.1) is shown. In each sequence, TRL is boxed, the terminal nucleotides are shaded black, and conserved regions AnTn and γ (which are present near the left and right termini, respectively) are underlined. Ellipses denote the remainder of the genome.
Table 2.
Tandem reiterations in EHV-8 genomes.
Table 3.
Amino acid sequence identity of genes of the Irish EHV-8 strains to their homologues in EHV-1 and EHV-9.
Fig 4.
Phylogenetic tree of concatenated amino acid sequences of ORF3-ORF76 from the Irish equid herpesvirus 8 (EHV-8) strains, EHV-8 strain Wh and other equid alphaherpesvirus strains.
The tree was constructed by using the maximum likelihood method based on the JTT matrix-based model [30], and is drawn to scale. Bootstrap values from 1000 replicates are shown as percentages at major nodes.