Fig 1.
Venn diagram [84] showing tBLASTx (E-value <10−10) pair-wise ortholog matches of the E. varivestis to the gut transcriptomes to the characterized gut transcriptomes.
(A) four arthropod species with diverse feeding strategies (Pectinophora gossypiella, Haemaphysalis flava, Periplaneta Americana, Periplaneta americana); and (B) three herbivore Coleopteran beetles (Leptinotarsa decemlineata, Chrysomela tremulae, Gastrophysa viridula).
Fig 2.
BLASTx top ortholog matches to organisms within the NCBI non-redundant (nr) database for the subset of 992 Epilachna varivestis contigs encoding putative secreted proteins with a significant BLASTx match (E-value <10−3).
Only organisms with ≥10 matches are shown.
Fig 3.
Distribution of Epilachna varivestis contigs encoding putative secreted proteins among functional categories.
Bars indicate the proportion of genes in each category: Number of genes in each category is given beside each bar. Percentages do not total to 100 as not all categories are shown.
Fig 4.
Distribution of Epilachna varivestis contigs encoding putative secreted proteins involved in metabolism.
Table 1.
Impact of regurgitant on transmission of a beetle-borne virus (Bean pod mottle virus, BPMV) and a non-beetle transmissible virus (Soybean mosaic virus, SMV) using two different leaf inoculation techniques (mechanical, M and gross wounding, G).
For each inoculum/virus combination, 30 experimental plants were assayed. The percentage of virus-infected plants is shown and the number of infected plants is indicated in parenthesis. Statistical significance between treatments was tested using a Marascuilo procedure (see methods), different superscript letters denote statistically different treatments (P < 0.05).