Fig 1.
Voiding behavior in testosterone-induced BPH rats.
The rat BPH model was generated by testosterone injections for 4 weeks after castration. Sham: sham operated + olive oil injection; BPH: castration + testosterone propionate injection (dissolved in olive oil, 25 mg/kg, once daily). (A) Prostate (containing the ventral, lateral and dorsal lobes) weight and (B) prostate weight to body weight ratios (PW/BW) of sham and BPH rats. H&E stains of dorsal lobe (C and D) and ventral lobe in shame rats and BPH rats (E and F). (G) Micturition frequency, (H) mean voided volume and (I) total voided volume were measured in sham and BPH rats in the first 2 hours after water load (30 ml/kg). **P < 0.01, ***P < 0.001 versus sham control (Student t test). Scale bar, 50μm.
Fig 2.
Effects of silodosin on the micturition parameters in testosterone-induced BPH rats.
Twenty minutes after oral administration of silodosin, distilled water (30 ml/kg) were given orally, and the micturition parameters of BPH rats were measured for the first 2 hours after water load. (A) Micturition frequency, (B) mean voided volume and (C) total voided volume were measured in a metabolic cage. *P < 0.05, **P < 0.01 and ***P < 0.001 versus vehicle control (One-way ANOVA test).
Fig 3.
Effects of silodosin on the micturition parameters in BOO rats.
BOO rat model was established by partial ligature of the proximal urethra. Voiding behavior was studied after 4 weeks of recovery. Twenty minutes after oral administration of silodosin, distilled water (30 ml/kg) were given orally. (A) Micturition frequency, (B) mean voided volume and (C) total voided volume were measured for the first 2 hours after water load. ##P < 0.01 versus sham control (Student t test). *P < 0.05, **P < 0.01 and ***P < 0.001 versus vehicle control (One-way ANOVA test).
Fig 4.
Voiding behavior in testosterone-induced BPH dogs.
Prostatic dimensions were obtained with ultrasonic diagnosis instrument before castration (pre-BPH) and 8 weeks after testosterone propionate administration (BPH). (A-D) Representative ultrasound images of the prostates of a pre-BPH dog (A and B) and a testosterone-induced BPH dog (C and D). Line 1, line 2 and line 3 represented the prostate length, width and thickness, respectively. Dog prostate volume = length × width × thickness. The prostate volume (E) and prostate volume to body weight ratio (F) of pre-BPH and testosterone-induced BPH dogs. H&E stains of prostate in pre-BPH and BPH dogs (G and H). (I) Micturition frequency, (J) mean voided volume and (K) total voided volume in the first 2 hours after water load were measured at 8 weeks after the induction of BPH. *P < 0.05 and ***P < 0.001 versus pre-BPH control (Student t test). Scale bar, 50μm.
Fig 5.
Effects of silodosin on the micturition parameters in testosterone-induced BPH dogs.
Twenty minutes after oral administration of silodosin, distilled water (30 ml/kg) were given orally. (A) Micturition frequency, (B) mean voided volume and (C) total voided volume in the first 2 hours after water load were measured in a metabolic cage. *P < 0.05, ***P < 0.001 versus vehicle control (One-way ANOVA test).