Table 1.
Primer characteristics.
Fig 1.
Effect of SLM on APAP-induced liver injury.
(A) ALT and (B) AST serum content; (C) necrotic lesions (dashed lines) in the liver determined by hematoxylin and eosin staining, original magnification x 100. Data are presented as a mean ± SEM, n = 7. **p < 0.005, ***p < 0.001 APAP vs control; #p < 0.05, ###p < 0.001 APAP+SLM vs APAP.
Fig 2.
Effect of APAP and SLM on RIP-3 expression.
The liver homogenate was separated by SDS-ELFO, immunoblotted and the proteins of interest were detected using specific antibody.
Fig 3.
Effect of APAP and SLM on CYP2E1 activity in vivo (A), mRNA expression (B), protein content (C) and CYPE1 activity in vitro (D, E). Results represent mean ± SEM, n = 7. *p < 0.05, **p < 0.01, ***p < 0.001 APAP vs control. #p < 0.05, ##p < 0.01 APAP + SLM vs APAP.
Fig 4.
Effect of APAP and SLM on reactive oxygen species production from submitochondrial particles in vitro.
(A) ROS production from NADH -/+ AA; (B) ROS production from succinate -/+ AA. Results are expressed as a percent of basal levels (without substrate). Data are presented as a mean ± SEM, n = 7. *p < 0.05, ***p < 0.001 APAP vs control (succinate + AA); † p < 0.05 APAP + SLM vs APAP (only succinate); #p < 0.05, ## p < 0.01 APAP + SLM vs APAP (succinate + AA).
Fig 5.
Effect of APAP and SLM on oxidative stress in the liver.
(A) GSH content in liver homogenate; (B) GSSG content in liver homogenate; (C) HO-1 mRNA expression; (D) p-JNK and t-JNK protein expression in liver homogenate. Results are expressed as mean ± SEM, n = 7. *p < 0.05, **p < 0.01, ***p < 0.001 APAP group vs control; #p < 0.05, ## p < 0.01, ### p < 0.001 APAP + SLM group vs APAP group.
Fig 6.
Nitrotyrosine formation in liver.
Nitrotyrosine staining is shown as dark brown colored sections. Blue color identified healthy hepatocytes. Original magification x 100.
Fig 7.
Effect of APAP and SLM on lipid peroxidation in the liver.
Data are given as mean ± SEM, n = 7. *p < 0.05 APAP vs control; #p < 0.05, ##p < 0.01 APAP + SLM vs APAP.
Fig 8.
Effect of APAP and SLM on neutrophil infiltration.
Tissue sections were stained with hematoxylin and eosin, black arrows indicate infiltrating neutrophiles. Original magnification x 600.
Fig 9.
Effect of APAP and SLM on mRNA expression of selected inflammatory markers.
(A) Ccl2; (B) Ccr2; (C) Ccl3; (D) TNFα; (E) IL 1β; (F) IL 12; (G) iNOS. Data are expressed as a fold change related to the untreated (control) group. Values are given as a mean ± SEM, n = 7. *p < 0.05, **p < 0.01, ***p < 0.001 APAP vs control; #p < 0.05, ##p < 0.01, ###p < 0.001 APAP+SLM vs APAP.