Table 1.
Clinical characteristics of pediatric oncology patients.
Fig 1.
Fecal bacterial diversity among pediatric oncology cohort.
Barcharts represent the percent relative abundances of genus-level taxa, as determined by 16S rRNA gene sequencing. All taxa with mean abundances across all subjects of less than 1% were aggregated into the “Other” category.
Table 2.
Associations between clinical/demographic variables and gastrointestinal microbiome1.
Table 3.
Differences in gastrointestinal microbiome between patient types1.
Fig 2.
Alpha biodiversity indices in association with patient characteristics.
Richness, evenness, and complexity (i.e., Shannon Diversity) were inferred from 16S rRNA sequence datasets through rarefaction and replicate resampling. P-values across all groups were ascertained by ANOVA. Abbreviations for patient types are detailed in the text. “Subsequent C. diff” compared subjects who had no history of C. difficile infection with those who recorded an infection following admission. Similarly, “Subsequent BSI” compared subjects who had no history of bloodstream infection with those who recorded an infection following admission. ALL: acute lymphoblastic leukemia; AML: acute myelogenous leukemia; BMT: bone marrow transplant; Heme: hematology; Solid: neuroblastoma, rhabdomyosarcoma, Wilm’s tumor, retinoblastoma, osteogenic sarcoma, ovarian endodermal sinus tumor, etc.
Fig 3.
Manhattan plots display the p-values for each OTU along the x-axis, following Kruskal-Wallis tests of relative abundance. P-values are plotted on the y-axis following–log10 transformation. OTUs that are significant in one or more statistical test are listed to the right of the plots. Horizontal lines denote p-values of 0.1, 0.05 or 0.01. Taxa that are either discuss in the text or that had p < 0.01 are labeled on each plot.
Table 4.
Fecal 16S rRNA gene abundance of pathogens cultured from bloodstream infections occurring following admission.