Table 1.
The detailed list of ingredients in low fat, high animal fat, and high fructose diets.
Fig 1.
Plots of spectrum (black dots) measured from the LFD (top), HAFD (middle), and HFruD (bottom) fed SV40 mice, and corresponding fitted the spectrum (color lines) with a combination of Gaussian and Lorentz functions.
1: Olefin (−CH = CH−) at 5.4 ppm, 2: Water (H2O) at 4.65 ppm, 3: Diallyl (= CH−CH2−CH =) at 2.8 ppm, 4: α-Methylene to carboxyl at 2.3 ppm, 5: Allyl (−CH2−CH = CH−) at 2.1 ppm, 6: Methylene (−CH2−) at 1.3 ppm, 7: Methyl (−CH3) at 0.9 ppm. The locations of 1 mm3 ROIs (white squares) used for the PRESS pulse sequence are shown in the insets T2W images. The right panel shows examples of histological images from inguinal mammary glands.
Fig 2.
The box-plots of poly-unsaturated fatty acids (PUFA), mono-unsaturated fatty acids (MUFA), and saturated fatty acids (SFA) calculated from spectra acquired from the LFD, HAFD, and HFruD fed SV40 mice inguinal mammary glands.
The squares (□) indicate mean, and the asterisks (*) indicate the upper and lower limits of the data.
Fig 3.
The box-plots of mammary lipid quantity calculated from spectra acquired from the LFD, HAFD, and HFruD fed SV40 mice inguinal mammary glands.
The square (□) indicate mean, and the asterisks (*) indicate the upper and lower limits of the data.
Fig 4.
Example of T2W images (top row), water peak height (PH) images (maps) (middle row), and fat peak height (PH) images (maps) (bottom row) for LFD (left panel), HAFD (middle panel), and HFruD (right panel) fed SV40 mice.
The color bar indicates the normalized signal intensity values, which were obtained by dividing the average of water peak height intensity over the muscle ROI.