Fig 1.
Schematic illustration of the XN-BF gating algorithm.
(A) Area 1 includes the aggregated cells due to the high forward scatter width signals that implies the long “time of flight” of the cells passing though the laser beam in the flow-cell. The HF-BF cells gated in Area 1 are considered as the Rule1 positive cells. The horizontal axis indicates forward scatter pulse width signal and the vertical axis shows forward scatter signal in the 2D scattergram. In the 3D scattergram, X-axis shows side scatter signal, Y-axis is forward scatter signal and Z-axis indicates forward scatter pulse width signal. (B) Area 2 includes the non-aggregated HF-BF cell showing relatively low fluorescent signal intensity, which are defined as the Rule 2 positive cells. The horizontal axis indicates forward scatter signal and the vertical axis shows side fluorescence signal in the 2D scattergram. In the 3D scattergram, X-axis indicates side scatter signal, Y-axis shows side fluorescence signal and Z-axis is forward scatter signal. The gating illustrated based on the plotting WDF channel dot plots.
Table 1.
Characteristics of BF samples.
Table 2.
Performance of examinations in hematology laboratory in the detection of BF malignancy compared with cytological diagnosis.
Table 3.
Sysmex XN-BF data and performance of examinations in hematology laboratory to detect malignancy.
Fig 2.
Representative analyses of body fluid samples by the XN-BF gating algorithm and photomicrographs of their corresponding cytospins.
Representative XN-BF scattergrams (Rule1 and Rule2) and pictures of cytospin slides of the exact same samples (May-Grunwald-Giemsa, original magnification x10 and x50). (A) PE with adenocarcinoma cells (sample #4, Table 2). Rule 1 positive and Rule 2 positive by the XN-BF gating algorithm. (B) PE with adenocarcinoma cells (sample #13, Table 2). Rule 1 positive, but Rule 2 negative by the XN-BF gating algorithm. (C) PE with malignant mesothelioma cells (sample #16, Table 2) Rule 1 negative, but Rule 2 positive by the XN-BF gating algorithm. (D) PE with adenocarcinoma cells (sample #18, Table 2) interpreted as negative for malignancy by the XN-BF gating algorithm (Rule 1 negative and Rule 2 negative).