Fig 1.
Analysis of photoinitiator absorbance spectral overlap.
Overlap of (a) SA-FITC and (b) SA-EITC with a Thorlabs collimated LED emitting green light. Lamp spectra obtained from manufacturer.
Fig 2.
Specific polymerization of photoinitiator tagged microarrays.
(A) Grayscale fluorescence scanner image of SA-EITC bound to biotin-BSA printed microarrays. Scan at 50 db gain with 532 nm excitation and 551 nm PMT detection with a 25 nm bandpass filter. (B) Grayscale fluorescence scanner image of SA-FITC bound to biotin-BSA printed microarrays. Scan at 50 db gain with 532 nm excitation and 551 nm PMT detection with a 25 nm bandpass filter. (C) Bright field optical microscopy example image of a microarray sample tagged with SA-FITC after photopolymerization at 30 mW/cm2 for 10 minutes.
Fig 3.
Comparative analysis of polymer gelation thickness vs. photoinitiator density for SA-FITC and SA-EITC tagged microarray samples.
Analysis was conducted at cell-compatible formulation and reaction conditions consisting of 420 mM PEG-diacrylate, 21 mM triethanol amine, 35 mM vinyl pyrrolidone, in phosphate buffered media (pH = 7.5) with a constant reaction time of 10 minutes.
Fig 4.
Comparison of polymer gelation thickness vs. photoinitiator density with cell compatible and unconstrained formulations and FITC photoinitiation.
All samples were irradiated with 20mW/cm2 of green light (530 nm LED lamp, ThorLabs) for 10 minutes. Cell compatible = 420 mM PEG-diacrylate, 21 mM triethanol amine, 35 mM vinyl pyrrolidone, in phosphate buffered media (pH = 7.5). Unconstrained = 420 mM PEG-diacrylate, 210 mM triethanol amine, 35 mM vinyl pyrrolidone in deionized water.
Fig 5.
Polymerization of A549 cells using an eosin or fluorescein photoinitiator targeted to EGFR and PEGDA3500 as a monomer.
(a) Fluorescence image of A549 cells coated with red fluorescent hydrogel using an eosin photoinitiator. (b) Fluorescence image of A549 cells coated with red fluorescent hydrogel using a flurescein photoinitiator. (c) Viability of A549 cells determined using MTT assay at various stages of polymerization (n = 4).