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Fig 1.

Relative abundance of the core bacterial taxa associated with laboratory-reared adult Ae. aegypti mosquitoes.

The circular plot depicts the abundance of the core bacterial taxa identified by crossing the indices of the f-OTUs per group for the experimental Ae. aegypti groups: sugar-fed (blue); blood-fed (red); ZIKV-infected blood-fed (maroon); Gravid (purple); and ZIKV-infected gravid (lilac). The bacterial taxa and experimental groups are indicated next to their ribbons on the perimeter of the circle. The width of the ribbon and the position of the f-OTUs from the bottom to the top (on each segment) reflect the abundance of the bacterial taxa and their presence within each group.

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Fig 1 Expand

Fig 2.

Raw abundance of the core bacterial communities per Ae. aegypti group.

The presence of arbitrarily selected core f-OTUs per group is shown as stacked bars to explore the dynamic shifts exhibited within each experimental group, which is represented by a color as indicated in the figure.

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Fig 2 Expand

Fig 3.

Exclusive bacterial components associated with the Ae. aegypti group.

The circular plot depicts the relative abundance of the exclusive bacterial taxa that were identified per experimental Ae. aegypti group: sugar-fed (blue), blood-fed (red), ZIKV-infected blood-fed (maroon), Gravid (purple), and ZIKV-infected gravid (lilac). The names of the bacterial f-OTUs and experimental groups are indicated next to their ribbons on the perimeter of the circle. The width of the ribbon and the position of the f-OTUs from the bottom to the top (on each segment) reflect the abundance of each bacterial taxon and their presence in each group.

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Fig 3 Expand

Fig 4.

Raw abundance of the exclusive bacterial taxa present in the studied groups of adult mosquitoes.

The raw abundance of the arbitrarily filtered f-OTUs that appeared to be present exclusively in the studied groups is shown as stacked bars revealing how community diversity is altered in the experimental groups. Each condition is represented by a color as indicated.

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Fig 4 Expand

Fig 5.

CCA triplot with explanatory biological effectors.

The distribution of the experimental groups along the axes, and their orientation regarding the arrow vectors suggest that exposure to blood (red and maroon) and ZIKV infection (maroon and lilac) had an impact on the signature profiles of the bacterial community. The CCA plot, along its two axes (CCA1 = 31.15% and CCA2 = 24.25%), could explain 55.40% of the matrix variance. Experimental groups: sugar-fed (blue), blood-fed (red), ZIKV-infected blood-fed (maroon), Gravid (violet), and ZIKV-infected gravid (lilac).

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Fig 5 Expand