Fig 1.
A) Magnetite synthesis with type 1 green rust as intermediate. a) Type 1 green rust—singlecore MNP. b) Fe3O4/γ-Fe2O3 -MCP. c) CMD-Fe3O4/γ-Fe2O3 -MCP. B) Carboxymethyl dextran sodium salt (CMD).
Table 1.
Difference between MCP 1 and MCP 2.
Fig 2.
TEM images (scale bar: 50nm) on the left and corresponding SAED patterns (scale bar: 2 nm-1) on the right of (a) MCP 1, (b) MCP 2–1 and (c) MCP 2–2. For magnified TEM images of MCP 1 and MCP 2–2 see supplement.
Fig 3.
TEM size distributions of (a) MCP 1, (b) MCP 2–1 and (c) MCP 2–2 based on measurement of 200 MCP in each case. The y-axis of the histogram represents the number of particles.
Table 2.
Compilation of important properties of the MCP determined by magnetic measurement, TEM and DLS.
Fig 4.
Mass magnetization M as a function of applied external field H measured for MCP 1, MCP2-1 and MCP2-2 with a SQUID at 295°C.
Table 3.
Fit parameters obtained from analysis of the M(H).
Here Ms is the saturation magnetization while β, dv2, σ2, and μ2 denote the volume fraction, the diameter of mean volume, the dispersion parameter and the mean magnetic moment of the MNP of the second mode of the fitted bimodal lognormal distribution. M3 is the MPS-amplitude at 3rd harmonics.
Fig 5.
MPS data of MCP 1, MCP 2–1 and MCP 2–2 in comparison with Resovist® (10 mT, 25kHz).
Data are plotted as magnetic moment (normalized for iron content) versus frequency. Only odd harmonics are shown, and lines have been added to guide the eye.
Fig 6.
In vivo MRI at 1.5 Tesla: T1-weighted 3D gradient-echo (GRE) fast low angle shot sequence.
a–Image without administration of MCP 1; b–Image obtained 2 minutes after administration of 0.1 mmol Fe/kg of MCP 1.
Fig 7.
T1 weighted MRI blood half-life measurement of MCP 1 (50 and 100μmol Fe/kg) at 1.5 Tesla: T1-weighted 3D gradient-echo (GRE) fast low angle shot sequence.
Fig 8.
In vivo MPI image of vena cava of a rat after i.v. bolus administration of 0.05 mmol Fe/kg (0.1 ml) Resovist (left) and MCP 2–2 (right) respectively.
For both image reconstructions the same parameters were used. 3D volume of field of view (FOV) with a size of 28 x 28 x 14 mm3 is shown.
Fig 9.
In vivo MRI image at 1.0 Tesla (ICON MRI, Bruker): T1-weighted 2D gradient-echo (GRE) fast low angle shot sequence overlaid with 3D volume in vivo MPI image of Fig 8 of the same rat.