Table 1.
Definitions of experimental conditions and time points of sample collection.
Fig 1.
Time points of possible sample collection.
Schematic view of samples that potentially could have been collected over the time course of the day depending on the animals’ implantation and training status.
Fig 2.
Decline of salivary cortisol concentration [ng/ml] over the day (binned for 60 minutes). The typically observed pronounced after-awakening peak and associated circadian decline during the morning hours [11,12] does not show prominently in our data, likely because we collected samples after the animals were awake for at least one hour (at or after 8 am).
Table 2.
Overview of collected samples.
Fig 3.
Cortisol values for applied fluid protocol, implant cleaning, and training session.
(A) Mean ± standard error (SEM) of cortisol concentrations averaged across the mean values of each animal for a given condition. N = number of animals contributing to the value. (B) Cortisol concentrations (ln-transformed) of saliva samples collected across conditions as used in the GAMM. Each circle depicts the mean value of ln-transformed cortisol values per individual, with the size of the circles indicating the number of samples for that data point. Horizontal black bars indicate the predicted values for cortisol from the model. To aid interpretation of displayed model estimates, collection times were z-transformed. See also Tables 2 and 3, respectively. * indicate p < 0.0001, n.s. represent p > 0.05. Percentages shown represent magnitudes of significant increases in mean cortisol values plotted in (A).
Table 3.
Effect of implant cleaning and access to fluid on salivary cortisol.
Table 4.
Effect of chaired training sessions on salivary cortisol.